Galectin-1 stimulates monocyte chemotaxis via the p44/42 MAP kinase pathway and a pertussis toxin-sensitive pathway.

Malik, Reshad K J, Ghurye, Rohit R, Lawrence-Watt, Diana J and Stewart, Helen J S (2009) Galectin-1 stimulates monocyte chemotaxis via the p44/42 MAP kinase pathway and a pertussis toxin-sensitive pathway. Glycobiology, 19 (12). pp. 1402-7. ISSN 1460-2423

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Abstract

Galectin-1, the prototype of a family of beta-galactoside-binding proteins, has been implicated in a wide variety of biological processes. Data presented herein show that galectin-1 stimulates monocyte migration in a dose-dependent manner but is not chemotactic for macrophages. Galectin-1-induced monocyte chemotaxis is blocked by lactose and inhibited by an anti-galectin-1 antibody but not by nonspecific antibodies. Furthermore, galectin-1-mediated monocyte migration was significantly inhibited by MEK inhibitors in a rapid, time-dependent manner suggesting that MAP kinase pathways are involved in galectin-1. Migration was also almost completely blocked by pertussis toxin implying G-protein involvement in the galectin-1-induced chemotaxis. These results demonstrate a role for galectin-1 in monocyte chemotaxis which differs from galectin-3 in that macrophages are nonresponsive. Furthermore, our observations suggest that galectin-1 may be involved in chemoattraction at sites of inflammation in vivo and may contribute to disease processes such as atherosclerosis.

Item Type: Article
Keywords: galectin-1, monocytes
Schools and Departments: Brighton and Sussex Medical School > Clinical and Laboratory Investigation
Brighton and Sussex Medical School > Clinical and Experimental Medicine
Subjects: Q Science > QD Chemistry > QD0241 Organic chemistry > QD0415 Biochemistry
Q Science > QH Natural history > QH0301 Biology
Depositing User: Helen Stewart
Date Deposited: 18 Aug 2011 13:39
Last Modified: 01 Sep 2017 10:14
URI: http://sro.sussex.ac.uk/id/eprint/7042
Google Scholar:7 Citations
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