Identification, structure and mode of action of a new regulator of the Helicobacter pylori HP0525 ATPase

Hare, Stephen, Fischer, Wolfgang, Williams, Robert, Terradot, Laurent, Bayliss, Richard, Haas, Rainer and Waksman, Gabriel (2007) Identification, structure and mode of action of a new regulator of the Helicobacter pylori HP0525 ATPase. EMBO Journal, 26 (23). pp. 4926-4934. ISSN 0261-4189

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Abstract

Helicobacter pylori is one of the world's most successful human pathogens causing gastric ulcers and cancers. A key virulence factor of H. pylori is the Cag pathogenicity island, which encodes a type IV secretion system. HP0525 is an essential component of the Cag system and acts as an inner membrane associated ATPase. HP0525 forms double hexameric ring structures, with the C‐terminal domains (CTDs) forming a closed ring and the N‐terminal domains (NTDs) forming a dynamic, open ring. Here, the crystal structure of HP0525 in complex with a fragment of HP1451, a protein of previously unknown function, is reported. The HP1451 construct consists of two domains similar to nucleic acid‐binding domains. Two HP1451 molecules bind to the HP0525 NTDs on opposite sides of the hexamer, locking it in the closed form and forming a partial lid over the HP0525 chamber. From the structure, it is suggested that HP1451 acts as an inhibitory factor of HP0525 to regulate Cag‐mediated secretion, a suggestion confirmed by results of in vitro ATPase assay and in vivo pull‐down experiments.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Depositing User: Stephen Hare
Date Deposited: 21 Sep 2017 14:41
Last Modified: 21 Sep 2017 14:41
URI: http://sro.sussex.ac.uk/id/eprint/70293
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