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Structural insights into the retroviral DNA integration apparatus

journal contribution
posted on 2023-06-09, 08:02 authored by Peter Cherepanov, Geodele N Maertens, Stephen Hare
Retroviral replication depends on successful integration of the viral genetic material into a host cell chromosome. Virally encoded integrase, an enzyme from the DDE(D) nucleotidyltransferase superfamily, is responsible for the key DNA cutting and joining steps associated with this process. Insights into the structural and mechanistic aspects of integration are directly relevant for the development of antiretroviral drugs. Recent breakthroughs have led to biochemical and structural characterization of the principal integration intermediates revealing the tetramer of integrase that catalyzes insertion of both 3' viral DNA ends into a sharply bent target DNA. This review discusses the mechanism of retroviral DNA integration and the mode of action of HIV-1 integrase strand transfer inhibitors in light of the recent visualization of the prototype foamy virus intasome, target DNA capture and strand transfer complexes.

History

Publication status

  • Published

Journal

Current Opinion in Structural Biology

ISSN

0959-440X

Publisher

Elsevier

Issue

2

Volume

21

Page range

249-256

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2017-09-21

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