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Structural insights into the retroviral DNA integration apparatus
journal contribution
posted on 2023-06-09, 08:02 authored by Peter Cherepanov, Geodele N Maertens, Stephen HareRetroviral replication depends on successful integration of the viral genetic material into a host cell chromosome. Virally encoded integrase, an enzyme from the DDE(D) nucleotidyltransferase superfamily, is responsible for the key DNA cutting and joining steps associated with this process. Insights into the structural and mechanistic aspects of integration are directly relevant for the development of antiretroviral drugs. Recent breakthroughs have led to biochemical and structural characterization of the principal integration intermediates revealing the tetramer of integrase that catalyzes insertion of both 3' viral DNA ends into a sharply bent target DNA. This review discusses the mechanism of retroviral DNA integration and the mode of action of HIV-1 integrase strand transfer inhibitors in light of the recent visualization of the prototype foamy virus intasome, target DNA capture and strand transfer complexes.
History
Publication status
- Published
Journal
Current Opinion in Structural BiologyISSN
0959-440XPublisher
ElsevierExternal DOI
Issue
2Volume
21Page range
249-256Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2017-09-21Usage metrics
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