Synthesis and characterization of a novel chemically designed (Globo)3–DTPA–KLH antigen

Hajmohammadi, Mehdi, Siadat, Seyed Davar, Ghorbani, Masoud, Shafiee Ardestani, Mehdi, Teimourian, Shahram, Asgari, Vahid, Ahangari Cohan, Reza, Hajmohammadi, Mostafa, Hajmohammadi, Akram, Behzadi, Ramezan, Rajabnezhad, Saeid and Namvar Asl, Nabiollah (2014) Synthesis and characterization of a novel chemically designed (Globo)3–DTPA–KLH antigen. Drug Design, Development and Therapy, 9. pp. 217-239. ISSN 1177-8881

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Abstract

In recent years, many experiments have been conducted for the production and evaluation of anticancer glycoconjugated vaccines in developed countries and many achievements have been accomplished with Globo H derivatives. In the current experiment, a new chemically designed triplicate version of (Globo H)3–diethylenetriamine pentaacetic acid (DTPA)–KLH antigen was synthesized and characterized. Immunization with (Globo H)3-DTPA-KLH, a hexasaccharide that is a member of a family of antigenic carbohydrates that are highly expressed in various types of cancers conjugated with DTPA and KLH protein, induced a high level of antibody titer along with an elevated level of IL-4 in mice. Treatment of tumors with the collected sera from immunized mice decreased the tumor size in nude mice as well. None of the immunized mice illustrated any sign of tumor growth after injection of MCF-7 cells compared to the control animals. These findings, based on the newly presented structure of the Globo H antigen, lend exciting and promising evidence for clinical advancement in the development of a therapeutic vaccine in the future.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Research Centres and Groups: Pharma Supply Chains and Healthcare Technology
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Depositing User: Saeid Rajabnezhad
Date Deposited: 12 Sep 2017 08:09
Last Modified: 12 Sep 2017 08:20
URI: http://sro.sussex.ac.uk/id/eprint/70085

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