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Synthesis and characterization of a novel chemically designed (Globo)3–DTPA–KLH antigen

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posted on 2023-06-09, 07:53 authored by Mehdi Hajmohammadi, Seyed Davar Siadat, Masoud Ghorbani, Mehdi Shafiee Ardestani, Shahram Teimourian, Vahid Asgari, Reza Ahangari Cohan, Mostafa Hajmohammadi, Akram Hajmohammadi, Ramezan Behzadi, Saeid Rajabnezhad, Nabiollah Namvar Asl
In recent years, many experiments have been conducted for the production and evaluation of anticancer glycoconjugated vaccines in developed countries and many achievements have been accomplished with Globo H derivatives. In the current experiment, a new chemically designed triplicate version of (Globo H)3–diethylenetriamine pentaacetic acid (DTPA)–KLH antigen was synthesized and characterized. Immunization with (Globo H)3-DTPA-KLH, a hexasaccharide that is a member of a family of antigenic carbohydrates that are highly expressed in various types of cancers conjugated with DTPA and KLH protein, induced a high level of antibody titer along with an elevated level of IL-4 in mice. Treatment of tumors with the collected sera from immunized mice decreased the tumor size in nude mice as well. None of the immunized mice illustrated any sign of tumor growth after injection of MCF-7 cells compared to the control animals. These findings, based on the newly presented structure of the Globo H antigen, lend exciting and promising evidence for clinical advancement in the development of a therapeutic vaccine in the future.

History

Publication status

  • Published

File Version

  • Published version

Journal

Drug Design, Development and Therapy

ISSN

1177-8881

Publisher

Dove Medical Press

Volume

9

Page range

217-239

Department affiliated with

  • Biochemistry Publications

Research groups affiliated with

  • Pharma Supply Chains and Healthcare Technology Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2017-09-12

First Open Access (FOA) Date

2017-09-12

First Compliant Deposit (FCD) Date

2017-09-12

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