SMC complexes differentially compact mitotic chromosomes according to genomic context

Schalbettertter, S A, Goloborodko, A, Fudenberg, G, Belton, J M, Miles, C, Yu, M, Dekker, J, Mirny, L and Baxter, J (2017) SMC complexes differentially compact mitotic chromosomes according to genomic context. Nature Cell Biology, 19 (9). pp. 1071-1080. ISSN 1465-7392

[img] PDF - Accepted Version
Restricted to SRO admin only until 21 February 2018.

Download (6MB)

Abstract

Structural maintenance of chromosomes (SMC) protein complexes are key determinants of chromosome conformation. Using Hi-C and polymer modelling, we study how cohesin and condensin, two deeply conserved SMC complexes, organize chromosomes in the budding yeast Saccharomyces cerevisiae. The canonical role of cohesin is to co-align sister chromatids, while condensin generally compacts mitotic chromosomes. We find strikingly different roles for the two complexes in budding yeast mitosis. First, cohesin is responsible for compacting mitotic chromosome arms, independently of sister chromatid cohesion. Polymer simulations demonstrate that this role can be fully accounted for through cis-looping of chromatin. Second, condensin is generally dispensable for compaction along chromosome arms. Instead, it plays a targeted role compacting the rDNA proximal regions and promoting resolution of peri-centromeric regions. Our results argue that the conserved mechanism of SMC complexes is to form chromatin loops and that distinct SMC-dependent looping activities are selectively deployed to appropriately compact chromosomes.

Item Type: Article
Keywords: Chromosome structure mitosis condensin cohesin SMC
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Research Centres and Groups: Genome Damage and Stability Centre
Subjects: Q Science > QR Microbiology > QR0001 General
Depositing User: Jon Baxter
Date Deposited: 07 Sep 2017 13:34
Last Modified: 07 Sep 2017 13:36
URI: http://sro.sussex.ac.uk/id/eprint/70028

View download statistics for this item

📧 Request an update
Project NameSussex Project NumberFunderFunder Ref
How does Condensin mediate topological change during mitosis?G0865Bbsrc-Biotechnology & Biological Sciences Research CouncilUnset