Comparison of Phenacoccus solenopsis specimens from different regions of Pakistan using COI molecular barcoding (Hemiptera: Pseudococcidae)

Badshah, Hayat, Ullah, Farman, Zada, Hayat, Zeb, Qamar, Shah, Ruidar A, Calatayud, Paul-André and Crickmore, Neil (2017) Comparison of Phenacoccus solenopsis specimens from different regions of Pakistan using COI molecular barcoding (Hemiptera: Pseudococcidae). Annales de la Societe Entomologique de France. ISSN 0037-9271

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Abstract

Because correct identification of insects is crucial for pest management involving chemical or biological control agents, we have used a molecular approach to identify and characterize specimens of the cotton pest Phenacoccus solenopsis Tinsley (Sternorrhyncha: Pseudococcidae) present in different regions of Pakistan. The specimens were analyzed through DNA sequence analysis of their mitochondrial COI (mtCOI) gene using an improved procedure that could distinguish between the pest and its associated parasitoid. Our analysis showed no variation among the mealybug specimens from different geographical locations of Pakistan and confirmed that this is the same species and haplotype that is infesting cotton plants in other parts of Asia. This information will assist in the development of biological control programs against P. solenopsis in Pakistan and other Asian countries.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Subjects: Q Science
Q Science > QL Zoology
Q Science > QL Zoology > QL0360 Invertebrates
Q Science > QL Zoology > QL0360 Invertebrates > QL0434 Arthropoda
Q Science > QL Zoology > QL0360 Invertebrates > QL0434 Arthropoda > QL0463 Insects
Depositing User: Neil Crickmore
Date Deposited: 21 Aug 2017 09:44
Last Modified: 10 Sep 2017 20:40
URI: http://sro.sussex.ac.uk/id/eprint/69889

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