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Regulation of P2X purinergic receptor signalling by cholesterol

chapter
posted on 2023-06-09, 07:27 authored by Ruth Murrell-LagnadoRuth Murrell-Lagnado
P2X receptors are cation-selective channels that are activated by the binding of extracellular ATP. They have a high permeability to Ca2+, Na+ and K+, and are expressed widely throughout the nervous, immune, cardiovascular, skeletal, gastrointestinal, respiratory, and endocrine systems. Seven mammalian subtypes of P2X receptor subunits have been identified, P2X1-7, and those that function as homotrimeric receptors (P2X1, 2, 3, 4 and 7) are targeted to lipid rafts, although they show limited resistance to solubilisation by Triton-X-100. Recent crystal structures of P2X3 and P2X4 receptors have provided considerable high-resolution information about the architecture of this family of receptors and yet the molecular details of how they are regulated by cholesterol are unknown. Currents mediated by the P2X1-4 receptors are either inhibited or relatively insensitive to cholesterol depletion but there is no clear evidence to support the direct binding of cholesterol to these receptors. In contrast, the activity of the low affinity, pro-inflammatory P2X7 receptor is potentiated by cholesterol depletion and regions within the proximal C-terminus play an important role in coupling changes in cholesterol to the gating of the pore. Based upon our understanding of the lipid signalling events that are triggered downstream of P2X7 receptor activation, a change in the levels of cholesterol may contribute to the sensitization of receptor currents and the dilation of the pore, that occurs following prolonged, high level stimulation. This chapter focuses on the regulation of P2X7 receptor signalling by cholesterol and our current understanding of the mechanisms that underlie this.

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Current Topics in Membranes

ISSN

1063-5823

Publisher

Elsevier

Volume

80

Page range

211-232

Book title

Sterol regulation of ion transport mechanisms

Series

Current topics in membranes

Department affiliated with

  • Neuroscience Publications

Full text available

  • No

Peer reviewed?

  • Yes

Editors

Irena Levitan

Legacy Posted Date

2017-07-27

First Compliant Deposit (FCD) Date

2017-07-27

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