Ephrin A/EphA controls the rostral turning polarity of a lateral commissural tract in chick hindbrain

Zhu, Yan, Guthrie, Sarah and Murakami, Fujio (2006) Ephrin A/EphA controls the rostral turning polarity of a lateral commissural tract in chick hindbrain. Development, 133 (19). pp. 3837-3846. ISSN 0950-1991

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Abstract

Most post-crossing commissural axons turn into longitudinal paths to make synaptic connections with their targets. Mechanisms that control their rostrocaudal turning polarity are still poorly understood. We used the hindbrain as a model system to investigate the rostral turning of a laterally located commissural tract, identified as the caudal group of contralateral cerebellar-projecting second-order vestibular neurons (cC-VC). We found that the caudal hindbrain possessed a graded non-permissive/repulsive activity for growing cC-VC axons. This non-permissiveness/repulsion was in part mediated by glycosyl-phosphatidylinositol (GPI)-anchored ephrin A. We further demonstrated that ephrin A2 was distributed in a caudal-high/rostral-low gradient in the caudolateral hindbrain and cC-VC axons expressed EphA receptors. Finally, perturbing ephrin A/EphA signalling both in vitro and in vivo led to rostrocaudal pathfinding errors of post-crossing cC-VC axons. These results suggest that ephrin A/EphA interactions play a key role in regulating the polarity of post-crossing cC-VC axons as they turn into the longitudinal axis.

Item Type: Article
Schools and Departments: School of Life Sciences > Neuroscience
Depositing User: Stephanie McGuire
Date Deposited: 11 Jul 2017 12:58
Last Modified: 11 Jul 2017 13:32
URI: http://sro.sussex.ac.uk/id/eprint/69172

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