PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast

Daigaku, Yasukazu, Etheridge, Thomas J, Nakazawa, Yuka, Nakayama, Mayumi, Watson, Adam T, Miyabe, Izumi, Ogi, Tomoo, Osborne, Mark A and Carr, Antony M (2017) PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast. PLoS Genetics, 13 (5). e1006789. ISSN 1553-7390

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Abstract

PCNA ubiquitylation on lysine 164 is required for DNA damage tolerance. In many organisms PCNA is also ubiquitylated in unchallenged S phase but the significance of this has not been established. Using Schizosaccharomyces pombe, we demonstrate that lysine 164 ubiquitylation of PCNA contributes to efficient DNA replication in the absence of DNA damage. Loss of PCNA ubiquitylation manifests most strongly at late replicating regions and increases the frequency of replication gaps. We show that PCNA ubiquitylation increases the proportion of chromatin associated PCNA and the co-immunoprecipitation of Polymerase δ with PCNA during unperturbed replication and propose that ubiquitylation acts to prolong the chromatin association of these replication proteins to allow the efficient completion of Okazaki fragment synthesis by mediating gap filling.

Item Type: Article
Schools and Departments: School of Life Sciences > Chemistry
School of Life Sciences > Sussex Centre for Genome Damage and Stability
Research Centres and Groups: Genome Damage and Stability Centre
Depositing User: Paula Amiet-West
Date Deposited: 08 May 2017 15:09
Last Modified: 12 Sep 2017 03:26
URI: http://sro.sussex.ac.uk/id/eprint/67639

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Project NameSussex Project NumberFunderFunder Ref
Replication fork stability and fork restartG0745MRC-MEDICAL RESEARCH COUNCILG1100074-E01/1
Single Molecule Imaging of the DNA Damage Response in Live CellsG0250EUROPEAN UNION268788