journal.pgen.1006789.pdf (8.69 MB)
PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast
Version 2 2023-06-12, 08:40
Version 1 2023-06-09, 06:04
journal contribution
posted on 2023-06-12, 08:40 authored by Yasukazu Daigaku, Thomas Etheridge, Yuka Nakazawa, Mayumi Nakayama, Adam WatsonAdam Watson, Izumi Miyabe, Tomoo Ogi, Mark Osborne, Antony CarrAntony CarrPCNA ubiquitylation on lysine 164 is required for DNA damage tolerance. In many organisms PCNA is also ubiquitylated in unchallenged S phase but the significance of this has not been established. Using Schizosaccharomyces pombe, we demonstrate that lysine 164 ubiquitylation of PCNA contributes to efficient DNA replication in the absence of DNA damage. Loss of PCNA ubiquitylation manifests most strongly at late replicating regions and increases the frequency of replication gaps. We show that PCNA ubiquitylation increases the proportion of chromatin associated PCNA and the co-immunoprecipitation of Polymerase d with PCNA during unperturbed replication and propose that ubiquitylation acts to prolong the chromatin association of these replication proteins to allow the efficient completion of Okazaki fragment synthesis by mediating gap filling.
Funding
Replication fork stability and fork restart; G0745; MRC-MEDICAL RESEARCH COUNCIL; G1100074-E01/1
Single Molecule Imaging of the DNA Damage Response in Live Cells; G0250; EUROPEAN UNION; 268788
History
Publication status
- Published
File Version
- Published version
Journal
PLoS GeneticsISSN
1553-7390Publisher
Public Library of ScienceExternal DOI
Issue
5Volume
13Article number
e1006789Department affiliated with
- Chemistry Publications
Research groups affiliated with
- Genome Damage and Stability Centre Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2017-05-08First Open Access (FOA) Date
2017-05-16First Compliant Deposit (FCD) Date
2017-05-08Usage metrics
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