The use of chromatin precipitation coupled to DNA sequencing (ChIP-Seq) for the analysis of Zta binding to the human and EBV genome

Godfrey, Anja, Ramasubramanyan, Sharada and Sinclair, Alison J (2017) The use of chromatin precipitation coupled to DNA sequencing (ChIP-Seq) for the analysis of Zta binding to the human and EBV genome. In: Minarovits, Janos and Niller, Hans Helmut (eds.) Epstein Barr virus: methods and protocols. Methods in molecular biology, 1532 . Springer, New York, pp. 191-206. ISBN 9781493966530

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Abstract

Determining which components of the transcription machinery associate with the viral and cellular genome, and how this changes at specific stages of the viral life cycle is paramount to understanding how the distinct transcriptional programs associated with primary infection, latency, and disease are established and how they are reprogrammed during initiation and execution of the viral lytic replication cycle. Chromatin precipitations linked to next generation DNA sequencing (ChIP-Seq) allow for the interactions of proteins with DNA to be mapped across both viral and cellular genomes. This can be applied to viral and cellular transcription factors, coactivators and corepressors, modified histones, and modulators of chromatin.

Item Type: Book Section
Schools and Departments: School of Life Sciences > Biochemistry
Research Centres and Groups: Gene Expression Research Group
Subjects: Q Science
Q Science > QR Microbiology
Q Science > QR Microbiology > QR0180 Immunology
Q Science > QR Microbiology > QR0355 Virology
Depositing User: Alison Sinclair
Date Deposited: 07 Apr 2017 09:35
Last Modified: 07 Apr 2017 09:35
URI: http://sro.sussex.ac.uk/id/eprint/67272
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