De novo design of a biologically active amyloid

Gallardo, Rodrigo, Ramakers, Meine, De Smet, Frederik, Claes, Filip, Khodaparast, Ladan, Khodaparast, Laleh, Couceiro, José R, Langenberg, Tobias, Siemons, Maxime, Nyström, Sofie, Young, Laurence J, Laine, Romain F, Young, Lydia, Radaelli, Enrico, Benilova, Iryna, Kumar, Manoj, Staes, An, Desager, Matyas, Beerens, Manu, Vandervoort, Petra, Luttun, Aernout, Gevaert, Kris, Bormans, Guy, Dewerchin, Mieke, Van Eldere, Johan, Carmeliet, Peter, Vande Velde, Greetje, Verfaillie, Catherine, Kaminski, Clemens F, De Strooper, Bart, Hammarström, Per, Nilsson, K Peter R, Serpell, Louise, Schymkowitz, Joost and Rousseau, Frederic (2016) De novo design of a biologically active amyloid. Science, 354 (6313). aah4949. ISSN 0036-8075

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Abstract

Most human proteins possess amyloidogenic segments, but only about 30 are associated with amyloid-associated pathologies, and it remains unclear what determines amyloid toxicity. We designed vascin, a synthetic amyloid peptide, based on an amyloidogenic fragment of vascular endothelial growth factor receptor 2 (VEGFR2), a protein that is not associated to amyloidosis. Vascin recapitulates key biophysical and biochemical characteristics of natural amyloids, penetrates cells, and seeds the aggregation of VEGFR2 through direct interaction. We found that amyloid toxicity is observed only in cells that both express VEGFR2 and are dependent on VEGFR2 activity for survival. Thus, amyloid toxicity here appears to be both protein-specific and conditional—determined by VEGFR2 loss of function in a biological context in which target protein function is essential.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Research Centres and Groups: Dementia Research Group
Depositing User: Louise Serpell
Date Deposited: 04 Apr 2017 10:49
Last Modified: 11 Sep 2017 23:33
URI: http://sro.sussex.ac.uk/id/eprint/67258

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