Specialized interfaces of Smc5/6 control hinge stability and DNA association

Alt, Aaron, Dang, Hung Q, Wells, Owen S, Polo, Luis M, Smith, Matt A, McGregor, Grant A, Welte, Thomas, Lehmann, Alan R, Pearl, Laurence H, Murray, Johanne M and Oliver, Antony W (2017) Specialized interfaces of Smc5/6 control hinge stability and DNA association. Nature Communications, 8. p. 14011. ISSN 2041-1723

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Abstract

The Structural Maintenance of Chromosomes (SMC) complexes: cohesin, condensin and Smc5/6 are involved in the organization of higher-order chromosome structure—which is essential for accurate chromosome duplication and segregation. Each complex is scaffolded by a specific SMC protein dimer (heterodimer in eukaryotes) held together via their hinge domains. Here we show that the Smc5/6-hinge, like those of cohesin and condensin, also forms a toroidal structure but with distinctive subunit interfaces absent from the other SMC complexes; an unusual ‘molecular latch’ and a functional ‘hub’. Defined mutations in these interfaces cause severe phenotypic effects with sensitivity to DNA-damaging agents in fission yeast and reduced viability in human cells. We show that the Smc5/6-hinge complex binds preferentially to ssDNA and that this interaction is affected by both ‘latch’ and ‘hub’ mutations, suggesting a key role for these unique features in controlling DNA association by the Smc5/6 complex.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: Q Science > Q Science (General)
Related URLs:
Depositing User: Antony Oliver
Date Deposited: 31 Jan 2017 15:39
Last Modified: 07 Mar 2017 17:22
URI: http://sro.sussex.ac.uk/id/eprint/66519

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