A wide field time resolved fluorescent lifetime imaging microscope

Birch, P, Moore, L, Li, X, Phillips, R and Conneely, T (2016) A wide field time resolved fluorescent lifetime imaging microscope. In: Photon 16, 5-8 September 2016, Leeds, UK.

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Abstract

Fluorescence lifetime imaging microscopy (FLIM) is a tool that has become of important use to many biologists and medical researchers. It allows for the imaging of fluorescent markers within cells and as well their decay lifetime information. This lifetime information yields useful information about the chemical properties of the local region surrounding the fluorophore marker. It is therefore desirable to be able to measure this information and localise it to produce either a 2D or 3D image stack of the lifetimes and intensity information. Two major problems must be overcome to achieve good resolution, both spatially and temporally. There must be a method to provide optical depth sectioning and the imaging must be fast. Sectioning can be done with confocal or two photon microscopes however, these are point scanning devices and are relatively slow.
We will detail a new microscope system based on light sheet illumination that uses a micro channel plate (MCP) device called a Capacitive Division Imaging Readout (CDIR) which has been developed by Photek Ltd. The device uses an array of capacitors to move the charge site from the MCP to four pre-amplifiers and time- over-threshold discriminators. This camera has the ability to image photons as well as measure the arrival time, enabling high speed FLIM imaging of biological samples. The optical sectioning is achieved by using a diffraction limited sheet of light, that illuminates the sample from the side in the plane orthogonal to the objectives optical axis.
We will present the first results taken from the microscope.

Item Type: Conference or Workshop Item (Paper)
Schools and Departments: School of Engineering and Informatics > Engineering and Design
Subjects: Q Science > QC Physics > QC0350 Optics. Light
Q Science > QR Microbiology
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Depositing User: Philip Birch
Date Deposited: 29 Sep 2016 09:54
Last Modified: 29 Sep 2016 09:54
URI: http://sro.sussex.ac.uk/id/eprint/63704
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