Gli2a protein localization reveals a role for Iguana/DZIP1 in primary ciliogenesis and a dependence of Hedgehog signal transduction on primary cilia in the zebrafish

Kim, Hyejeong Rosemary, Richardson, Joanna, van Eeden, Freek and Ingham, Philip W (2010) Gli2a protein localization reveals a role for Iguana/DZIP1 in primary ciliogenesis and a dependence of Hedgehog signal transduction on primary cilia in the zebrafish. BMC Biology, 8 (65). ISSN 1741-7007

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Abstract

BACKGROUND:
In mammalian cells, the integrity of the primary cilium is critical for proper regulation of the Hedgehog (Hh) signal transduction pathway. Whether or not this dependence on the primary cilium is a universal feature of vertebrate Hedgehog signalling has remained contentious due, in part, to the apparent divergence of the intracellular transduction pathway between mammals and teleost fish.

RESULTS:
Here, using a functional Gli2-GFP fusion protein, we show that, as in mammals, the Gli2 transcription factor localizes to the primary cilia of cells in the zebrafish embryo and that this localization is modulated by the activity of the Hh pathway. Moreover, we show that the Igu/DZIP1protein, previously implicated in the modulation of Gli activity in zebrafish, also localizes to the primary cilium and is required for its proper formation.

CONCLUSION:
Our findings demonstrate a conserved role of the primary cilium in mediating Hedgehog signalling activity across the vertebrate phylum and validate the use of the zebrafish as a representative model for the in vivo analysis of vertebrate Hedgehog signalling.

Item Type: Article
Keywords: Gli2, Hedgehog signalling, Zebrafish
Schools and Departments: School of Life Sciences > Biochemistry
Subjects: Q Science > QL Zoology > QL0951 Embryology
Depositing User: Joanna Richardson
Date Deposited: 28 Jul 2016 08:38
Last Modified: 09 Mar 2017 06:15
URI: http://sro.sussex.ac.uk/id/eprint/62132

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