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A wide field fluorescence lifetime imaging system using a light sheet microscope

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posted on 2023-06-09, 00:52 authored by Phil BirchPhil Birch, Lamar Moore, Xiaofei Li, Roger Phillips, Rupert YoungRupert Young, Chris ChatwinChris Chatwin
Fluorescence lifetime imaging microscopy (FLIM) has allowed scientists to discern information about the chemical properties of biological processes and has become a vital tool in the life sciences and medical research communities. Measuring the spatial lifetime distribution of the fluorophores as well as the intensity distribution enables users to discern vital information about the chemical environment. It however, remains challenging and often involves slow scanning. We present a new microscope system based on light sheet illumination that uses a micro channel plate (MCP) device called a Capacitive Division Imaging Readout (CDIR) which has been developed by Photek Ltd. The device uses an array of capacitors to move the charge site from the MCP to four pre-amplifiers and time-over-threshold discriminators. This camera has the ability to image photons as well as measure the arrival time, enabling high speed FLIM imaging of biological samples.

History

Publication status

  • Published

File Version

  • Accepted version

Presentation Type

  • paper

Event name

SPIE Photonics Europe

Event location

Brussels, Belgium

Event type

conference

Event date

3rd-7th April 2016

Department affiliated with

  • Engineering and Design Publications

Full text available

  • Yes

Peer reviewed?

  • No

Legacy Posted Date

2016-04-13

First Open Access (FOA) Date

2016-04-13

First Compliant Deposit (FCD) Date

2016-04-12

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