Primase-polymerases are a functionally diverse superfamily of replication and repair enzymes

Guilliam, Thomas A, Keen, Benjamin A, Brissett, Nigel C and Doherty, Aidan J (2015) Primase-polymerases are a functionally diverse superfamily of replication and repair enzymes. Nucleic Acids Research, 43 (14). pp. 6651-6664. ISSN 0305-1048

[img] PDF - Published Version
Available under License Creative Commons Attribution.

Download (2MB)

Abstract

Until relatively recently, DNA primases were viewed simply as a class of proteins that synthesize short RNA primers requisite for the initiation of DNA replication. However, recent studies have shown that this perception of the limited activities associated with these diverse enzymes can no longer be justified. Numerous examples can now be cited demonstrating how the term 'DNA primase' only describes a very narrow subset of these nucleotidyltransferases, with the vast majority fulfilling multifunctional roles from DNA replication to damage tolerance and repair. This article focuses on the archaeo-eukaryotic primase (AEP) superfamily, drawing on recently characterized examples from all domains of life to highlight the functionally diverse pathways in which these enzymes are employed. The broad origins, functionalities and enzymatic capabilities of AEPs emphasizes their previous functional misannotation and supports the necessity for a reclassification of these enzymes under a category called primase-polymerases within the wider functional grouping of polymerases. Importantly, the repositioning of AEPs in this way better recognizes their broader roles in DNA metabolism and encourages the discovery of additional functions for these enzymes, aside from those highlighted here.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Aidan Doherty
Date Deposited: 15 Jan 2016 13:14
Last Modified: 07 Mar 2017 10:08
URI: http://sro.sussex.ac.uk/id/eprint/59196

View download statistics for this item

📧 Request an update