Identification and characterization of novel factors that act in the nonsense-mediated mRNA decay pathway in nematodes, flies and mammals

Casadio, Angela, Longman, Dasa, Hug, Nele, Delavaine, Laurent, Vallejos Baier, Raul, Alonso, Claudio R and Caceres, Javier F (2015) Identification and characterization of novel factors that act in the nonsense-mediated mRNA decay pathway in nematodes, flies and mammals. EMBO reports, 16 (1). pp. 71-78. ISSN 1469-221X

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Abstract

Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism that degrades mRNAs harboring premature termination codons (PTCs). We have conducted a genome-wide RNAi screen in Caenorhabditis elegans that resulted in the identification of five novel NMD genes that are conserved throughout evolution. Two of their human homologs, GNL2 (ngp-1) and SEC13 (npp-20), are also required for NMD in human cells. We also show that the C. elegans gene noah-2, which is present in Drosophila melanogaster but absent in humans, is an NMD factor in fruit flies. Altogether, these data identify novel NMD factors that are conserved throughout evolution, highlighting the complexity of the NMD pathway and
suggesting that yet uncovered novel factors may act to regulate this process.

Item Type: Article
Schools and Departments: School of Life Sciences > Neuroscience
Subjects: Q Science > QH Natural history > QH0301 Biology > QH0426 Genetics
Depositing User: Claudio Alonso
Date Deposited: 15 Jan 2016 12:49
Last Modified: 07 Mar 2017 03:53
URI: http://sro.sussex.ac.uk/id/eprint/59179

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The molecular regulation of Hox genes during animal developmentG0952WELLCOME TRUST098410/Z/12/Z