Induction of human regulatory T cells with bacterial superantigens

Caserta, Stefano, Taylor, Amanda L, Terrazzini, Nadia and Llewelyn, Martin J (2016) Induction of human regulatory T cells with bacterial superantigens. In: Brosnahan, Amanda J (ed.) Superantigens. Methods in Molecular Biology, 1396 . Springer New York, New York, pp. 181-206. ISBN 9781493933426

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Abstract

Regulatory T cells (Tregs) that suppress the activation of immune effector cells limit immunopathology and are fast emerging as therapeutic targets for autoimmune and cancer disease. Tools enabling Treg in vitro-induction, expansion, and characterization and manipulation will help future clinical developments. In this chapter, we describe in detail how to use bacterial superantigens to induce human Tregs efficiently from peripheral blood mononuclear cells. How to assess human Treg phenotype and suppressive capacity are also described. Technical details, variations, and alternative experimental conditions are provided.

Item Type: Book Section
Keywords: superantigen regulatory T cells human superantigen stimulation Treg phenotype suppression assay cell purification PBMC immunological methods
Schools and Departments: Brighton and Sussex Medical School > Clinical Medicine
Brighton and Sussex Medical School > Global Health and Infection
Subjects: R Medicine > RC Internal medicine > RC0109 Infectious and parasitic diseases
R Medicine > RC Internal medicine > RC0581 Specialties of internal medicine > RC0582 Immunological diseases
Depositing User: Stefano Caserta
Date Deposited: 23 Dec 2015 13:48
Last Modified: 31 Jul 2017 18:11
URI: http://sro.sussex.ac.uk/id/eprint/58903
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