Weller, G. R., Kysela, B., Roy, R., Tonkin, L. M., Scanlan, E., Della, M., Devine, S. K., Day, J. P., Wilkinson, A., d'Adda di Fagagna, F., Devine, K. M., Bowater, R. P., Jeggo, P. A., Jackson, S. P. and Doherty, A. J. (2002) Identification of a DNA nonhomologous end-joining complex in bacteria. Science, 297 (5587). pp. 1686-9.Full text not available from this repository.
In eukaryotic cells, double-strand breaks (DSBs) in DNA are generally repaired by the pathway of homologous recombination or by DNA nonhomologous end joining (NHEJ). Both pathways have been highly conserved throughout eukaryotic evolution, but no equivalent NHEJ system has been identified in prokaryotes. The NHEJ pathway requires a DNA end-binding component called Ku. We have identified bacterial Ku homologs and show that these proteins retain the biochemical characteristics of the eukaryotic Ku heterodimer. Furthermore, we show that bacterial Ku specifically recruits DNA ligase to DNA ends and stimulates DNA ligation. Loss of these proteins leads to hypersensitivity to ionizing radiation in Bacillus subtilis. These data provide evidence that many bacteria possess a DNA DSB repair apparatus that shares many features with the NHEJ system of eukarya and suggest that this DNA repair pathway arose before the prokaryotic and eukaryotic lineages diverged.
|Keywords:||Antigens, Nuclear Bacillus subtilis/ genetics Bacterial Proteins/metabolism Binding Sites DNA Damage DNA Helicases DNA Ligases/ metabolism DNA Repair DNA, Bacterial/ biosynthesis/metabolism DNA-Binding Proteins/metabolism Mutation Nuclear Proteins/metabolism Protein Binding Research Support, Non-U.S. Gov't Saccharomyces cerevisiae Proteins|
|Schools and Departments:||School of Life Sciences|
|Depositing User:||Aidan Doherty|
|Date Deposited:||27 Nov 2006|
|Last Modified:||30 Nov 2012 16:50|
|Google Scholar:||168 Citations|