A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus solfataricus

Dionne, I., Nookala, R. K., Jackson, S. P., Doherty, A. J. and Bell, S. D. (2003) A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus solfataricus. Molecular Cell, 11 (1). pp. 275-82. ISSN 1097-2765

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Abstract

The sliding clamp, PCNA, of the archaeon Sulfolobus solfataricus P2 is a heterotrimer of three distinct subunits (PCNA1, 2, and 3) that assembles in a defined manner. The PCNA heterotrimer, but not individual subunits, stimulates the activities of the DNA polymerase, DNA ligase I, and the flap endonuclease (FEN1) of S. solfataricus. Distinct PCNA subunits contact DNA polymerase, DNA ligase, or FEN1, imposing a defined architecture at the lagging strand fork and suggesting the existence of a preformed scanning complex at the fork. This provides a mechanism to tightly couple DNA synthesis and Okazaki fragment maturation. Additionally, unique subunit-specific interactions between components of the clamp loader, RFC, suggest a model for clamp loading of PCNA.

Item Type: Article
Keywords: Amino Acid Sequence Archaeal Proteins/chemistry/genetics/ metabolism DNA, Archaeal/genetics/metabolism DNA-Directed DNA Polymerase/metabolism Models, Molecular Molecular Sequence Data Proliferating Cell Nuclear Antigen/chemistry/genetics/ metabolism Protein Conformation Protein Subunits/metabolism Recombinant Fusion Proteins/metabolism Research Support, Non-U.S. Gov't Sequence Alignment Sulfolobus/ metabolism Two-Hybrid System Techniques
Depositing User: Aidan Doherty
Date Deposited: 27 Nov 2006
Last Modified: 30 Nov 2012 16:50
URI: http://sro.sussex.ac.uk/id/eprint/576
Google Scholar:122 Citations
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