Steric antisense inhibition of AMPA receptor Q/R editing reveals tight coupling to intronic editing sites and splicing

Penn, Andrew C, Balik, Ales and Greger, Ingo H (2013) Steric antisense inhibition of AMPA receptor Q/R editing reveals tight coupling to intronic editing sites and splicing. Nucleic Acids Research, 41 (2). pp. 1113-1123. ISSN 0305-1048

[img]
Preview
PDF - Published Version
Available under License Creative Commons Attribution.

Download (10MB) | Preview

Abstract

Adenosine-to-Inosine (A-to-I) RNA editing is a post-transcriptional mechanism, evolved to diversify the transcriptome in metazoa. In addition to wide-spread editing in non-coding regions protein recoding by RNA editing allows for fine tuning of protein function. Functional consequences are only known for some editing sites and the combinatorial effect between multiple sites (functional epistasis) is currently unclear. Similarly, the interplay between RNA editing and splicing, which impacts on post-transcriptional gene regulation, has not been resolved. Here, we describe a versatile antisense approach, which will aid resolving these open questions. We have developed and characterized morpholino oligos targeting the most efficiently edited site--the AMPA receptor GluA2 Q/R site. We show that inhibition of editing closely correlates with intronic editing efficiency, which is linked to splicing efficiency. In addition to providing a versatile tool our data underscore the unique efficiency of a physiologically pivotal editing site.

Item Type: Article
Schools and Departments: School of Life Sciences > Neuroscience
Subjects: Q Science
R Medicine > RC Internal medicine > RC0321 Neurosciences. Biological psychiatry. Neuropsychiatry
Depositing User: Andrew Penn
Date Deposited: 07 Oct 2015 09:20
Last Modified: 06 Mar 2017 14:50
URI: http://sro.sussex.ac.uk/id/eprint/57002

View download statistics for this item

📧 Request an update