IL-7 is superior to IL-2 for ex vivo expansion of tumour-specific CD4(+) T cells

Caserta, Stefano, Alessi, Patrizia, Basso, Veronica and Mondino, Anna (2010) IL-7 is superior to IL-2 for ex vivo expansion of tumour-specific CD4(+) T cells. European Journal of Immunology, 40 (2). pp. 470-479. ISSN 0014-2980

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Abstract

It is well established that tumours hinder both natural and vaccine-induced tumour-specific CD4(+) T-cell responses. Adoptive T-cell therapy has the potential to circumvent functional tolerance and enhance anti-tumour protective responses. While protocols suitable for the expansion of cytotoxic CD8(+) T cells are currently available, data on tumour-specific CD4(+) T cells remain scarce. We report here that CD4(+) T cells sensitized to tumour-associated Ag in vivo, proliferate in vitro in response to IL-7 without the need for exogenous Ag stimulation and accumulate several folds while preserving a memory-like phenotype. Both cell proliferation and survival accounts for the outgrowth of tumour-sensitized T cells among other memory and naive lymphocytes following exposure to IL-7. Also IL-2, previously used to expand anti-tumour CTL, promotes tumour-specific CD4(+) T-cell accumulation. However, IL-7 is superior to IL-2 at preserving lymphocyte viability, in vitro and in vivo, maintaining those properties, that are required by helper CD4(+) T cells to confer therapeutic efficacy upon transplantation in tumour-bearing hosts. Together our data support a unique role for IL-7 in retrieving memory-like CD4(+) T cells suitable for adoptive T-cell therapy.

Item Type: Article
Keywords: cytokine biology; tumour immunotherapy; T cells; adoptive cell therapy
Schools and Departments: Brighton and Sussex Medical School > Clinical and Experimental Medicine
Brighton and Sussex Medical School > Global Health and Infection
Subjects: Q Science > QR Microbiology > QR0180 Immunology
R Medicine > RM Therapeutics. Pharmacology > RM0270 Immunotherapy. Serotherapy
Depositing User: Stefano Caserta
Date Deposited: 30 Sep 2015 06:56
Last Modified: 21 Sep 2017 13:54
URI: http://sro.sussex.ac.uk/id/eprint/56876
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