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Estimation of fluorescence lifetimes via rotational invariance techniques

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journal contribution
posted on 2023-06-08, 22:22 authored by Hongqi Yu, Rebecca Saleeb, Paul Dalgarno, David Day-Uei Li
Estimation of signal parameters via rotational invariance techniques is a classical algorithm widely used in array signal processing for direction-of-arrival estimation of emitters. Inspired by this method, a new signal model and a new fluorescence lifetime estimation via rotational invariance techniques (FLERIT) were developed for multi-exponential fluorescence lifetime imaging (FLIM) experiments. The FLERIT only requires a few time bins of a histogram generated by a time-correlated single photon counting FLIM system, greatly reducing the data throughput from the imager to the signal processing units. As a non-iterative method, the FLERIT does not require initial conditions, prior information nor model selection that are usually required by widely used traditional fitting methods, including nonlinear least square methods or maximum likelihood methods. Moreover, its simplicity means it is suitable for implementations in embedded systems for real-time applications. FLERIT was tested on synthesized and experimental fluorescent cell data showing the potentials to be widely applied in FLIM data analysis.

History

Publication status

  • Published

File Version

  • Published version

Journal

IEEE Transactions on Biomedical Engineering

ISSN

0018-9294

Publisher

Institute of Electrical and Electronics Engineers

Issue

6

Volume

63

Page range

1292-1300

Department affiliated with

  • Engineering and Design Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2015-09-03

First Open Access (FOA) Date

2016-09-26

First Compliant Deposit (FCD) Date

2015-09-03

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