The GAR motif of 53BP1 is arginine methylated by PRMT1 and is necessary for 53BP1 DNA binding activity

Boisvert, F. M., Rhie, A., Richard, S. and Doherty, A. J. (2005) The GAR motif of 53BP1 is arginine methylated by PRMT1 and is necessary for 53BP1 DNA binding activity. Cell Cycle, 4 (12). pp. 1834-41.

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Abstract

The p53-binding protein 1 (53BP1) is rapidly recruited to sites of DNA double-strand breaks and forms characteristics nuclear foci, demonstrating its role in the early events of detection, signaling and repair of damaged DNA. 53BP1 contains a glycine arginine rich (GAR) motif of unknown function within its kinetochore-binding domain. Herein, we show that the GAR motif of 53BP1 is arginine methylated by protein arginine methyltransferase 1 (PRMT1), the same methyltransferase that methylates MRE11. 53BP1 contains asymmetric dimethylarginines (aDMA) within cells, as detected with methylarginine-specific antibodies. Amino acid substitution of the arginines within the GAR motif of 53BP1 abrogated binding to single and double-stranded DNA, demonstrating that the GAR motif is required for DNA binding activity of 53BP1. Fibroblast cells treated with methylase inhibitors failed to relocalize 53BP1 to sites of DNA damage and formed few gamma-H2AX foci, consistent with our previous data that MRE11 fails to relocalize to DNA damage sites in cells treated with methylase inhibitors. Our findings identify the GAR motif as a region required for 53BP1 DNA binding activity and as the site of methylation by PRMT1.

Item Type: Article
Keywords: Amino Acid Motifs Amino Acid Sequence Arginine/ metabolism Cells, Cultured DNA/ metabolism DNA Damage Fibroblasts/cytology Glycine/ metabolism Hela Cells Humans Intracellular Signaling Peptides and Proteins/ chemistry/ metabolism Methylation Molecular Sequence Data Mutation Phosphoproteins/ chemistry/ metabolism Protein Binding Protein Transport Protein-Arginine N-Methyltransferase/antagonists & inhibitors/ metabolism Repressor Proteins/antagonists & inhibitors/ metabolism Research Support, Non-U.S. Gov't
Schools and Departments: School of Life Sciences
Depositing User: Aidan Doherty
Date Deposited: 27 Nov 2006
Last Modified: 30 Nov 2012 16:50
URI: http://sro.sussex.ac.uk/id/eprint/564
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