The rate of X-ray-induced DNA double-strand break repair in the embryonic mouse brain is unaff ected by exposure to 50 Hz magnetic fi elds

Woodbine, Lisa, Haines, Jackie, Coster, Margaret, Barazzuol, Lara, Ainsbury, Elizabeth, Sienkiewicz, Zenon and Jeggo, Penny (2015) The rate of X-ray-induced DNA double-strand break repair in the embryonic mouse brain is unaff ected by exposure to 50 Hz magnetic fi elds. International Journal of Radiation Biology, 91 (6). pp. 495-499. ISSN 0955-3002

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Abstract

Following in utero exposure to low dose radiation
(10 – 200 mGy), we recently observed a linear induction of DNA
double-strand breaks (DSB) and activation of apoptosis in the
embryonic neuronal stem/progenitor cell compartment. No
signifi cant induction of DSB or apoptosis was observed following
exposure to magnetic fi elds (MF). In the present study, we
exploited this in vivo system to examine whether exposure to MF
before and after exposure to 100 mGy X-rays impacts upon DSB
repair rates.
Materials and methods : 53BP1 foci were quantifi ed following
combined exposure to radiation and MF in the embryonic neuronal
stem/progenitor cell compartment. Embryos were exposed
in utero to 50 Hz MF at 300 m T for 3 h before and up to 9 h after
exposure to 100 mGy X-rays. Controls included embryos exposed
to MF or X-rays alone plus sham exposures.
Results : Exposure to MF before and after 100 mGy X-rays did not
impact upon the rate of DSB repair in the embryonic neuronal
stem cell compartment compared to repair rates following radiation
exposure alone.
Conclusions : We conclude that in this sensitive system MF do not
exert any signifi cant level of DNA damage and do not impede
the repair of X-ray induced damage.

Item Type: Article
Keywords: X-rays , extremely low frequency magnetic fi elds (ELF-MF) , low dose radiation , DNA double-strand break repair , embryonic neuronal stem cells
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: Q Science > QD Chemistry > QD0241 Organic chemistry > QD0415 Biochemistry
Depositing User: Gee Wheatley
Date Deposited: 04 Aug 2015 12:11
Last Modified: 07 Mar 2017 07:16
URI: http://sro.sussex.ac.uk/id/eprint/55996

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