Broader implications of SILAC-based proteomics for dissecting signaling dynamics in cancer

Zhang, Hua, Xu, Yichen, Papanastasopoulos, Panos, Stebbing, Justin and Giamas, Georgios (2014) Broader implications of SILAC-based proteomics for dissecting signaling dynamics in cancer. Expert Review of Proteomics, 11 (6). pp. 713-731. ISSN 1478-9450

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Abstract

Large-scale transcriptome and epigenome analyses have been widely utilized to discover gene alterations implicated in cancer development at the genetic level. However, mapping of signaling dynamics at the protein level is likely to be more insightful and needed to complement massive genomic data. Stable isotope labeling with amino acids in cell culture (SILAC)-based proteomic analysis represents one of the most promising comparative quantitative methods that has been extensively employed in proteomic research. This technology allows for global, robust and confident identification and quantification of signal perturbations important for the progress of human diseases, particularly malignancies. The present review summarizes the latest applications of in vitro and in vivo SILAC-based proteomics in identifying global proteome/phosphoproteome and genome-wide protein-protein interactions that contribute to oncogenesis, highlighting the recent advances in dissecting signaling dynamics in cancer.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Subjects: Q Science > QH Natural history > QH0301 Biology
Depositing User: Georgios Giamas
Date Deposited: 07 Jul 2015 08:33
Last Modified: 07 Jul 2015 08:33
URI: http://sro.sussex.ac.uk/id/eprint/55212
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