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LATS2 is a modulator of estrogen receptor alpha

journal contribution
posted on 2023-06-08, 21:28 authored by Lei Cheng Lit, Susan Scott, Hua Zhang, Justin Stebbing, Andrew Photiou, Georgios GiamasGeorgios Giamas
BACKGROUND Estrogen Receptor a (ERa), a member of the nuclear receptor superfamily of transcription factors, plays a central role in breast cancer development. More than two-thirds of patients with breast cancer are ERa-positive; however, a proportion becomes resistant. Phosphorylation of ERa is one of the mechanisms associated with resistance to endocrine therapy. In a kinome screen, we have identified the large tumor suppressor homolog-2 (LATS2) as a potential kinase, acting on ERa. MATERIALS AND METHODS The role of LATS2 on activation of ERa transcription and its functional consequences was examined by various molecular and cellular biology techniques. RESULTS LATS2 co-localises with ERa in the nucleus. LATS2-silencing increases expression of ERa-regulated genes and inhibits proliferation. At the protein level, inhibition of LATS2 reduces the expression of cyclin-D1 and Nuclear Receptor Co-Repressor (NCoR) while increasing the expression of p27. CONCLUSION Identifying novel kinases which modulate ERa activity is relevant to therapeutics. LATS2 modulates ERa-regulated gene transcription, through direct and/or indirect interactions with ERa.

History

Publication status

  • Published

Journal

Anticancer Research

ISSN

0250-7005

Publisher

International Institute of Anticancer Research (IIAR)

Issue

1

Volume

33

Page range

53-63

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2015-07-07

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