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Translesion synthesis in mammalian cells

journal contribution
posted on 2023-06-07, 13:31 authored by Alan LehmannAlan Lehmann
DNA damage blocks the progression of the replication fork. In order to circumvent the damaged bases, cells employ specialized low stringency DNA polymerases, which are able to carry out translesion synthesis (TLS) past different types of damage. The five polymerases used in TLS in human cells have different substrate specificities, enabling them to deal with many different types of damaged bases. PCNA plays a central role in recruiting the TLS polymerases and effecting the polymerase switch from replicative to TLS polymerase. When the fork is blocked PCNA gets ubiquitinated. This increases its affinity for the TLS polymerases, which all have novel ubiquitin-binding motifs, thereby facilitating their engagement at the stalled fork to effect TLS.

History

Publication status

  • Published

Journal

Experimental Cell Research

ISSN

0014-4827

Publisher

Elsevier

Issue

14

Volume

312

Page range

2673-2676

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2006-11-23

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