The SMC5-6 DNA repair complex: Bridging of the SMC5-6 heads by the Kleisin, NSE4, and non-Kleisin subunits

Palecek, J., Vidot, S., Feng, M., Doherty, A. J. and Lehmann, A. R. (2006) The SMC5-6 DNA repair complex: Bridging of the SMC5-6 heads by the Kleisin, NSE4, and non-Kleisin subunits. Journal of Biological Chemistry, 2006 D (48). pp. 36952-36959. ISSN 0021-9258

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Abstract

Structural Maintenance of Chromosomes (SMC) proteins play fundamental roles in many aspects of chromosome organization and dynamics. The SMC complexes form unique structures with long coiled-coil arms folded at a hinge domain, so that the globular N- and C-terminal domains are brought together to form a "head". Within the Smc5/6 complex, we previously identified two subcomplexes containing Smc6-Smc5-Nse2 and Nse1-Nse3-Nse4. A third subcomplex containing Nse5 and 6 has also been identified recently. We present evidence that Nse4 is the kleisin component of the complex, which bridges the heads of Smc5 and 6. The C-terminal part of Nse4 interacts with the head domain of Smc5 and structural predictions for Nse4 proteins suggest similar motifs that are shared within the kleisin family. Specific mutations within a predicted winged helix motif of Nse4 destroy the interaction with Smc5. We propose that Nse4 and its orthologs form the d-kleisin subfamily. We further show that Nse3, as well as Nse5 and Nse6 also bridge the heads of Smc5 and 6. The Nse1-3-4 and Nse5-6 subcomplexes bind to the Smc5-6 heads domain at different sites.

Item Type: Article
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH0301 Biology
Depositing User: Alan Lehmann
Date Deposited: 06 Dec 2006
Last Modified: 07 Mar 2017 11:06
URI: http://sro.sussex.ac.uk/id/eprint/546

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