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The Scottish Structural Proteomics Facility: targets, methods and outputs
journal contribution
posted on 2023-06-15, 20:57 authored by Muse Oke, Lester G Carter, Kenneth A Johnson, Huanting Liu, Stephen A McMahon, Xuan Yan, Melina Kerou, Nadine D Weikart, Nadia Kadi, Md Arif Sheikh, Stefan Schmelz, Mark Dorward, Michal Zawadzki, Christopher Cozens, Helen Falconer, Helen Powers, Ian M Overton, C A Johannes van Niekerk, Xu Peng, Prakash Patel, Roger A Garrett, David Prangishvili, Catherine H Botting, Peter J Coote, David T F Dryden, Geoffrey J Barton, Ulrich Schwarz-Linek, Gregory L Challis, Garry L Taylor, Malcolm F White, James H NaismithThe Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. These structures and the methods harnessed to obtain them are reported here. This report reflects on the value of automation but also on the continued requirement for a high degree of scientific and technical expertise. The efficiency of the process poses challenges to the current paradigm of structural analysis and publication. In the 5 year period we published ten peer-reviewed papers reporting structural data arising from the pipeline. Nevertheless, the number of structures solved exceeded our ability to analyse and publish each new finding. By reporting the experimental details and depositing the structures we hope to maximize the impact of the project by allowing others to follow up the relevant biology.
History
Publication status
- Published
File Version
- Published version
Journal
Journal of Structural and Functional GenomicsISSN
1345-711XPublisher
Springer VerlagExternal DOI
Issue
2Volume
11Page range
167-180Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2015-05-07First Open Access (FOA) Date
2015-05-07First Compliant Deposit (FCD) Date
2015-05-07Usage metrics
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