Carbon dots (C-dots) from cow manure with impressive subcellular selectivity tuned by simple chemical modification

Barbosa, Cintya, Corrêa, José R., Medeiros, Gisele A, Barreto, Gabrielle, Magalhães, Kelly G, de Oliveira, Aline L, Spencer, John, Rodrigues, Marcelo O and Neto, Brenno A D (2015) Carbon dots (C-dots) from cow manure with impressive subcellular selectivity tuned by simple chemical modification. Chemistry - A European Journal, 21 (13). 5055 -5060. ISSN 0947-6539

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Abstract

Improved cellular selectivity toward nucleoli staining was achieved by simple chemical modification of carbon dots (C-dots) synthesized from waste carbon sources such as cow manure (or from glucose). The C-dots were characterized and functionalized (amine-passivated) with ethylenediamine affording amide bonds exhibiting bright green fluorescence. The new modified C-dots were successfully applied as selective live cell fluorescence imaging probes with impressive subcellular selectivity and selectively stain nucleoli in breast cancer cell lineages (MCF-7). The C-dots were also tested in four other cellular models showing the same cellular selection in live cell-imaging experiments.

Item Type: Article
Schools and Departments: School of Life Sciences > Chemistry
Depositing User: John Spencer
Date Deposited: 04 May 2015 07:15
Last Modified: 08 Mar 2017 08:03
URI: http://sro.sussex.ac.uk/id/eprint/53789

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