“Breaking up is hard to do”: the formation and resolution of sister chromatid intertwines

Baxter, Jonathan (2014) “Breaking up is hard to do”: the formation and resolution of sister chromatid intertwines. Journal of Molecular Biology, 427 (3). pp. 590-607. ISSN 0022-2836

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Abstract

The absolute necessity to resolve every intertwine between the two strands of the DNA double helix provides a massive challenge to the cellular processes that duplicate and segregate chromosomes. Although the overwhelming majority of intertwines between the parental DNA strands are resolved during DNA replication, there are numerous chromosomal contexts where some intertwining is maintained into mitosis. These mitotic sister chromatid intertwines (SCIs) can be found as; short regions of unreplicated DNA, fully replicated and intertwined sister chromatids—commonly referred to as DNA catenation—and as sister chromatid linkages generated by homologous recombination-associated processes. Several overlapping mechanisms, including intra-chromosomal compaction, topoisomerase action and Holliday junction resolvases, ensure that all SCIs are removed before they can prevent normal chromosome segregation. Here, I discuss why some DNA intertwines persist into mitosis and review our current knowledge of the SCI resolution mechanisms that are employed in both prokaryotes and eukaryotes, including how deregulating SCI formation during DNA replication or disrupting the resolution processes may contribute to aneuploidy in cancer.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: R Medicine
Depositing User: Catrina Hey
Date Deposited: 09 Apr 2015 15:29
Last Modified: 15 Nov 2017 15:05
URI: http://sro.sussex.ac.uk/id/eprint/53632

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Project NameSussex Project NumberFunderFunder Ref
How does Condensin mediate topological change during mitosis?G0865BBSRC-BIOTECHNOLOGY & BIOLOGICAL SCIENCES RESEARCH COUNCILBB/J018554/1
Understanding the mechanisms of termination of DNA replication in eukaryotes Fellow: BaxterG0222ROYAL SOCIETYUO090655