Tel1ATM dictates the replication timing of short yeast telomeres

Cooley, Carol, Dave, Anoushka, Garg, Mansi and Bianchi, Alessandro (2014) Tel1ATM dictates the replication timing of short yeast telomeres. EMBO Reports, 15 (10). pp. 1093-1101. ISSN 1469-221X

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Abstract

Telomerase action is temporally linked to DNA replication. Although yeast telomeres are normally late replicating, telomere shortening leads to early firing of subtelomeric DNA replication origins. We show that double‐strand breaks flanked by short telomeric arrays cause origin firing early in S phase at late‐replicating loci and that this effect on origin firing time is dependent on the Tel1ATM checkpoint kinase. The effect of Tel1ATM on telomere replication timing extends to endogenous telomeres and is stronger than that elicited by Rif1 loss. These results establish that Tel1ATM specifies not only the extent but also the timing of telomerase recruitment.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Depositing User: Mrs Mansi Garg
Date Deposited: 25 Mar 2015 13:27
Last Modified: 08 Mar 2017 18:10
URI: http://sro.sussex.ac.uk/id/eprint/53504

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Project NameSussex Project NumberFunderFunder Ref
Analysis of regulation telomerase action & telomore capping function by the telomeric complex (Fellow: Bianchi)G0129MRC-MEDICAL RESEARCH COUNCILG0701428
Mechanism of telomerase activation and inhibition at fission yeast telomeresG0247CANCER RESEARCH UKC28567/A12720