CarrAAM1419260428.pdf (3.33 MB)
A global profile of replicative polymerase usage
journal contribution
posted on 2023-06-08, 20:25 authored by Yasukazu Daigaku, Andrea KeszthelyiAndrea Keszthelyi, Carolin A Müller, Izumi Miyabe, Tony Brooks, Renata Retkute, Mike Hubank, Conrad A Nieduszynski, Antony CarrAntony CarrThree eukaryotic DNA polymerases are essential for genome replication. Polymerase (Pol) a–primase initiates each synthesis event and is rapidly replaced by processive DNA polymerases: Pol? replicates the leading strand, whereas Pold performs lagging-strand synthesis. However, it is not known whether this division of labor is maintained across the whole genome or how uniform it is within single replicons. Using Schizosaccharomyces pombe, we have developed a polymerase usage sequencing (Pu-seq) strategy to map polymerase usage genome wide. Pu-seq provides direct replication-origin location and efficiency data and indirect estimates of replication timing. We confirm that the division of labor is broadly maintained across an entire genome. However, our data suggest a subtle variability in the usage of the two polymerases within individual replicons. We propose that this results from occasional leading-strand initiation by Pold followed by exchange for Pol?.
Funding
Replication fork stability and fork restart; G0745; MRC-MEDICAL RESEARCH COUNCIL; G1100074-E01/1
ERC; 268788-SMI-DDR
History
Publication status
- Published
File Version
- Accepted version
Journal
Nature Structural and Molecular BiologyISSN
1545-9993Publisher
Nature Publishing GroupExternal DOI
Issue
3Volume
22Page range
192-198Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2015-03-25First Open Access (FOA) Date
2015-08-09First Compliant Deposit (FCD) Date
2015-03-25Usage metrics
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