A global profile of replicative polymerase usage

Daigaku, Yasukazu, Keszthelyi, Andrea, Müller, Carolin A, Miyabe, Izumi, Brooks, Tony, Retkute, Renata, Hubank, Mike, Nieduszynski, Conrad A and Carr, Antony M (2015) A global profile of replicative polymerase usage. Nature Structural and Molecular Biology, 22 (3). pp. 192-198. ISSN 1545-9993

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Abstract

Three eukaryotic DNA polymerases are essential for genome replication. Polymerase (Pol) α–primase initiates each synthesis event and is rapidly replaced by processive DNA polymerases: Polɛ replicates the leading strand, whereas Polδ performs lagging-strand synthesis. However, it is not known whether this division of labor is maintained across the whole genome or how uniform it is within single replicons. Using Schizosaccharomyces pombe, we have developed a polymerase usage sequencing (Pu-seq) strategy to map polymerase usage genome wide. Pu-seq provides direct replication-origin location and efficiency data and indirect estimates of replication timing. We confirm that the division of labor is broadly maintained across an entire genome. However, our data suggest a subtle variability in the usage of the two polymerases within individual replicons. We propose that this results from occasional leading-strand initiation by Polδ followed by exchange for Polɛ.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: R Medicine
Depositing User: Catrina Hey
Date Deposited: 25 Mar 2015 12:29
Last Modified: 14 Mar 2017 08:04
URI: http://sro.sussex.ac.uk/id/eprint/53501

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Project NameSussex Project NumberFunderFunder Ref
Replication fork stability and fork restartG0745MRC-MEDICAL RESEARCH COUNCILG1100074-E01/1
UnsetUnsetERC268788-SMI-DDR