Actions of n-alcohols on nicotinic acetylcholine receptor ion channels in cultured rat muscle cells

Murrell, R D and Haydon, D A (1991) Actions of n-alcohols on nicotinic acetylcholine receptor ion channels in cultured rat muscle cells. Annals of the New York Academy of Sciences, 625. pp. 365-374. ISSN 0077-8923

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Abstract

The effects of the n-alcohols from pentanol to dodecanol on nAChR channel function were resolved at the single channel level. ACh-activated channel activity was recorded from isolated membrane patches using the patch clamp method. The intermediate-chain alcohols (C5-C8) had two main effects: (1) They caused channel openings to be interrupted by brief shut or blocked periods, the duration of which was dependent on chain length of the alcohol but independent of concentration. (2) They caused a reduction in the duration of bursts of openings. The long-chain alcohols (C9-C11) produced only the second effect, and there was a decline in activity beyond undecanol. Results were consistent with a mechanism of channel blockade and were analyzed in terms of an open channel block model with a long-lived closed-blocked state beyond the blocked state. Affinity for the binding site increased with chain length up to octanol. The standard free energy per methylene group for adsorption to the site was calculated to be -3.3 kJ/mol, indicating the very hydrophobic nature of the site.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Subjects: Q Science > QH Natural history > QH0301 Biology
Depositing User: Tom Gittoes
Date Deposited: 25 Mar 2015 14:16
Last Modified: 25 Mar 2015 14:16
URI: http://sro.sussex.ac.uk/id/eprint/52633
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