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High-resolution icosahedral reconstruction: fulfilling the promise of cryo-electron microscopy

journal contribution
posted on 2023-06-08, 19:51 authored by Erika ManciniErika Mancini, Felix de Haas, Stephen D Fuller
Two recent papers have defined the secondary structure of the hepatitis B virus capsid using a combination of cryoelectron microscopy and icosahedral image reconstruction. These two papers do more than reveal a new fold for a virus protein; they herald a new era in which image reconstruction of single particles will provide reliable high-resolution structural information. In revealing the promise of these techniques to the structural biology community, their two papers should play a seminal role for single particle work, similar to that of the work of Unwin and Henderson [I] on bacteriorhodopsin in revealing the potential of electron microscopy of membrane protein crystals. Indeed, the success of these single particle methods owes much to the development of high-resolution techniques for two-dimensional crystals. This review will summarize some of the history of icosahedral reconstruction from cryo-electron micrographs, compare the two different approaches used to obtain the recent results and outline some of the challenges and promises for the future.

History

Publication status

  • Published

Journal

Structure

ISSN

0969-2126

Publisher

Elsevier

Issue

6

Volume

5

Page range

741-750

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2015-01-29

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