Supplanting crystallography or supplementing microscopy? A combined approach to the study of an enveloped virus

Mancini, E J and Fuller, S D (2000) Supplanting crystallography or supplementing microscopy? A combined approach to the study of an enveloped virus. Acta Crystallographica Section D: Biological Crystallography, 56 (10). pp. 1278-1287. ISSN 0907-4449

[img]
Preview
PDF (© International Union of Crystallography) - Published Version
Download (1MB) | Preview

Abstract

The recent advances in the resolution obtained by single-particle reconstructions from cryo-electron microscopy (cryo-EM) have led to an increase in studies that combine X-ray crystallographic results with those of electron microscopy (EM). Here, such a combination is described in the determination of the structure of an enveloped animal virus, Semliki Forest virus, at 9 Å resolution. The issues of model bias in determination of the structure, the definition of resolution in a single-particle reconstruction, the effect of the correction of the contrast-transfer function on the structure determined and the use of a high-resolution structure of a subunit in the interpretation of the structure of the complex are addressed.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Subjects: Q Science
Depositing User: Tom Gittoes
Date Deposited: 29 Jan 2015 10:19
Last Modified: 26 Mar 2017 16:38
URI: http://sro.sussex.ac.uk/id/eprint/52568

View download statistics for this item

📧 Request an update