Cryo-electron microscopy reveals the functional organization of an enveloped virus, Semliki Forest virus

Mancini, Erika J, Clarke, Mairi, Gowen, Brent E, Rutten, Twan and Fuller, Stephen D (2000) Cryo-electron microscopy reveals the functional organization of an enveloped virus, Semliki Forest virus. Molecular Cell, 5 (2). pp. 255-266. ISSN 1097-2765

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Abstract

Semliki Forest virus serves as a paradigm for membrane fusion and assembly. Our icosahedral reconstruction combined 5276 particle images from 48 cryo-electron micrographs and determined the virion structure to 9 Å resolution. The improved resolution of this map reveals an N-terminal arm linking capsid subunits and defines the spike-capsid interaction sites. It illustrates the paired helical nature of the transmembrane segments and the elongated structures connecting them to the spike projecting domains. A 10 Å diameter density in the fusion protein lines the cavity at the center of the spike. These clearly visible features combine with the variation in order between the layers to provide a framework for understanding the structural changes during the life cycle of an enveloped virus.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Subjects: Q Science
Depositing User: Tom Gittoes
Date Deposited: 29 Jan 2015 09:55
Last Modified: 29 Jan 2015 09:55
URI: http://sro.sussex.ac.uk/id/eprint/52567
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