A procedure for setting up high-throughput nanolitre crystallization experiments. II. Crystallization results

Brown, J, Walter, T S, Carter, L, Abrescia, N G A, Aricescu, A R, Batuwangala, T D, Bird, L E, Brown, N, Chamberlain, P P, Davis, S J, Dubinina, E, Endicott, J, Fennelly, J A, Gilbert, R J C, Harkiolaki, M, Hon, W-C, Kimberley, F, Love, C A, Mancini, E J, Manso-Sancho, R, Nichols, C E, Robinson, R A, Sutton, G C, Schueller, N, Sleeman, M C, Stewart-Jones, G B, Vuong, M, Welburn, J, Zhang, Z, Stammers, D K, Owens, R J, Jones, E Y, Harlos, K and Stuart, D I (2003) A procedure for setting up high-throughput nanolitre crystallization experiments. II. Crystallization results. Journal of Applied Crystallography, 36 (2). pp. 315-318. ISSN 0021-8898

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Abstract

An initial tranche of results from day-to-day use of a robotic system for setting up 100 nl-scale vapour-diffusion sitting-drop protein crystallizations has been surveyed. The database of over 50 unrelated samples represents a snapshot of projects currently at the stage of crystallization trials in Oxford research groups and as such encompasses a broad range of proteins. The results indicate that the nanolitre-scale methodology consistently identifies more crystallization conditions than traditional hand-pipetting-style methods; however, in a number of cases successful scale-up is then problematic. Crystals grown in the initial 100 nl-scale drops have in the majority of cases allowed useful characterization of x-ray diffraction, either in-house or at synchrotron beamlines. For a significant number of projects, full x-ray diffraction data sets have been collected to 3 Å resolution or better (either in-house or at the synchrotron) from crystals grown at the 100 nl scale. To date, five structures have been determined by molecular replacement directly from such data and a further three from scale-up of conditions established at the nanolitre scale.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Subjects: Q Science
Depositing User: Tom Gittoes
Date Deposited: 29 Jan 2015 11:33
Last Modified: 10 Mar 2017 15:42
URI: http://sro.sussex.ac.uk/id/eprint/52565

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