Semi-automated microseeding of nanolitre crystallization experiments

Walter, T S, Mancini, E J, Kadlec, J, Graham, S C, Assenberg, R, Ren, J, Sainsbury, S, Owens, R J, Stuart, D I, Grimes, J M and Harlos, K (2008) Semi-automated microseeding of nanolitre crystallization experiments. Acta Crystallographica Section F, 64 (1). pp. 14-18. ISSN 1744-3091

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Abstract

A simple semi-automated microseeding procedure for nanolitre crystallization experiments is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96-well crystallization plates, facilitating the incorporation of seeding into high-throughput crystallization pipelines. This basic microseeding procedure has been modified to include additive-screening and cross-seeding methods. Five examples in which these techniques have been used successfully are described. © International Union of Crystallography 2008.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Subjects: Q Science
Depositing User: Tom Gittoes
Date Deposited: 29 Jan 2015 15:37
Last Modified: 10 Mar 2017 03:10
URI: http://sro.sussex.ac.uk/id/eprint/52558

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