Concerted action of the PHD, chromo and motor domains regulates the human chromatin remodelling ATPase CHD4

Morra, Rosa, Lee, Benjamin M, Shaw, Heather, Tuma, Roman and Mancini, Erika J (2012) Concerted action of the PHD, chromo and motor domains regulates the human chromatin remodelling ATPase CHD4. FEBS Letters, 586 (16). pp. 2513-2521. ISSN 0014-5793

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Abstract

CHD4, the core subunit of the Nucleosome Remodelling and Deacetylase (NuRD) complex, is a chromatin remodelling ATPase that, in addition to a helicase domain, harbors tandem plant homeo finger and chromo domains. By using a panel of domain constructs we dissect their roles and demonstrate that DNA binding, histone binding and ATPase activities are allosterically regulated. Molecular shape reconstruction from small-angle X-ray scattering reveals extensive domain-domain interactions, which provide a structural explanation for the regulation of CHD4 activities by intramolecular domain communication. Our results demonstrate functional interdependency between domains within a chromatin remodeller. Crown Copyright © 2012 Published by Elsevier B.V. on behalf of Federation of European Biochemical society. All rights reserved.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Subjects: Q Science
Depositing User: Tom Gittoes
Date Deposited: 30 Jan 2015 10:41
Last Modified: 06 Mar 2017 22:57
URI: http://sro.sussex.ac.uk/id/eprint/52550

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