Human saphenous vein endothelial cells express a tetrodotoxin- resistant, voltage-gated sodium current

Gosling, Martin, Harley, Suzanne L, Turner, Robert J, Carey, Nessa and Powell, Janet T (1998) Human saphenous vein endothelial cells express a tetrodotoxin- resistant, voltage-gated sodium current. Journal of Biological Chemistry, 273 (33). pp. 21084-21090. ISSN 0021-9258

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Abstract

Whole-cell patch-clamp electrophysiological investigation of endothelial cells cultured from human saphenous vein (HSVECs) has identified a voltage- gated Na+ current with a mean peak magnitude of -595 ± 49 pA (n = 75). This current was inhibited by tetrodotoxin (TTX) in a concentration-dependent manner, with an IC50 value of 4.7 μM, suggesting that it was of the TTX- resistant subtype. An antibody directed against the highly conserved intracellular linker region between domains III and IV of known Na+ channel α-subunits was able to retard current inactivation when applied intracellularly. This antibody identified a 245-kDa protein from membrane lysates on Western blotting and positively immunolabeled both cultured HSVECs and intact venous endothelium. HSVECs were also shown by reverse transcription-polymerase chain reaction to contain transcripts of the hH1 sodium channel gene. The expression of Na+ channels by HSVECs was shown using electrophysiology and cell-based enzyme-linked immunosorbent assay to be dependent on the concentration and source of human serum. Together, these results suggest that TTX-resistant Na+ channels of the hill isoform are expressed in human saphenous vein endothelium and that the presence of these channels is controlled by a serum factor.

Item Type: Article
Schools and Departments: School of Life Sciences > Chemistry
Subjects: Q Science > QD Chemistry
Depositing User: Tom Gittoes
Date Deposited: 27 Jan 2015 12:23
Last Modified: 27 Jan 2015 12:23
URI: http://sro.sussex.ac.uk/id/eprint/52448
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