Methodology for profiling anti-androgen mixtures in river water using multiple passive samplers and bioassay-directed analyses

Liscio, Camilla, Abdul-Sada, Alaa, Al-Salhi, Raghad, Ramsey, Michael H. and Hill, Elizabeth M (2014) Methodology for profiling anti-androgen mixtures in river water using multiple passive samplers and bioassay-directed analyses. Water Research, 57. pp. 258-269. ISSN 0043-1354

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Abstract

The identification of endocrine disrupting chemicals in surface waters is challenging as they comprise a variety of structures which are often present at nanomolar concentrations and are temporally highly variable. Hence, a holistic passive sampling approach can be an efficient technique to overcome these limitations. In this study, a combination of 4 different passive samplers used for sampling polar (POCIS Apharm and POCIS Bpesticide) and apolar compounds (LDPE low density polyethylene membranes, and silicone strips) were used to profile anti-androgenic activity present in river water contaminated by a wastewater effluent. Extracts of passive samplers were analysed using HPLC fractionation in combination with an in vitro androgen receptor antagonist screen (YAS). Anti-androgenic activity was detected in extracts from silicone strips and POCIS A/B at (mean ± SD) 1.1 ± 0.1 and 0.55 ± 0.06 mg flutamide standard equivalents/sampler respectively, but was not detected in LDPE sampler extracts. POCIS samplers revealed higher selectivity for more polar anti-androgenic HPLC fractions compared with silicone strips. Over 31 contaminants were identified which showed inhibition of YAS activity and were potential anti-androgens, and these included fungicides, germicides, flame retardants and pharmaceuticals. This study reveals that passive sampling, using a combination of POCIS A and silicone samplers, is a promising tool for screening complex mixture of anti-androgenic contaminants present in surface waters, with the potential to identify new and emerging structures with endocrine disrupting activity.

Item Type: Article
Schools and Departments: School of Life Sciences > Chemistry
School of Life Sciences > Evolution, Behaviour and Environment
Subjects: T Technology > TD Environmental technology. Sanitary engineering > TD0172 Environmental pollution
Depositing User: Elizabeth Hill
Date Deposited: 23 Jan 2015 08:47
Last Modified: 23 Jan 2015 08:47
URI: http://sro.sussex.ac.uk/id/eprint/52404
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