Development of pH-sensitive insulin nanoparticles using Eudragit L100-55 and chitosan with different molecular weights

Jelvehgari, Mitra, Zakeri-Milani, Parvin, Siahi-Shadbad, Mohammad Reza, Loveymi, Badir Delf, Nokhodchi, Ali, Azari, Zahra and Valizadeh, Hadi (2010) Development of pH-sensitive insulin nanoparticles using Eudragit L100-55 and chitosan with different molecular weights. AAPS PharmSciTech, 11 (3). pp. 1237-1242. ISSN 1530-9932

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Abstract

Insulin is a polypeptide hormone and usually administered for treatment of diabetic patients subcutaneously. The aim of this study was to investigate the efficiency of enteric nanoparticles for oral delivery of insulin. Nanoparticles were formed by complex coacervation method using chitosan of various molecular weights. Nanoparticles were characterized by drug loading efficiency determination, particle size analysis, Scanning Electron Microscopy (SEM), Zeta potential and CD spectroscopy (Circular Dichrosim). The in vitro release studies were performed at pH 1.2 and 7.4. The drug loaded nanoparticles showed 3.38% of entrapment, loading efficiency of 30.56% and mean particle size of 199 nm. SEM studies showed that the nanoparticles are non-spherical. Zeta potential increased with increasing molecular weight of chitosan. The CD spectroscopy profiles indicated that the nano-encapsulation process did not significantly disrupt the internal structure of insulin; additionally, pH-sensitivity of nanoparticles was preserved and the insulin release was pH-dependent. These results suggest that the complex coacervation process using chitosan and Eudragit L100-55 polymers may provide a useful approach for entrapment of hydrophilic polypeptides without affecting their conformation. © 2010 American Association of Pharmaceutical Scientists.

Item Type: Article
Schools and Departments: School of Life Sciences > Chemistry
Subjects: Q Science > QD Chemistry
Depositing User: Tom Gittoes
Date Deposited: 21 Dec 2014 19:28
Last Modified: 21 Dec 2014 19:28
URI: http://sro.sussex.ac.uk/id/eprint/51794
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