Development and validation of a novel rp-hplc method for pharmacokinetic studies of gliclazide in rat

Talar, R, Arshosaz, J, Mostafavi, A and Nokhodchi, Ali (2011) Development and validation of a novel rp-hplc method for pharmacokinetic studies of gliclazide in rat. Farmacia, 59 (3). pp. 388-395. ISSN 0014-8237

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Abstract

A sensitive, simple and accurate reverse phase HPLC method was developed to determine plasma levels of gliclazide (GL) in rat. The method uses liquid-liquid extraction that is not expensive and ibuprofen as internal standard, that is easily available. Mean recovery for GL and internal standard was 80% and 82% respectively. Relative standard errors and CV% ranged from 0.47 to 8.99 and from 0.72 to 12.54% for intraday and interday HPLC injections, respectively. The limit of quantitation (LOQ) and the limit of detection (LOD) for GL in serum were 0.12 μg/mL and 0.06 μg/mL respectively. The developed HPLC method is a suitable and rapid method to be adopted for pharmacokinetic studies in rat and human.

Item Type: Article
Schools and Departments: School of Life Sciences > Chemistry
Subjects: Q Science > QD Chemistry
Depositing User: Tom Gittoes
Date Deposited: 18 Dec 2014 11:48
Last Modified: 18 Dec 2014 11:48
URI: http://sro.sussex.ac.uk/id/eprint/51781
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