Inhibition of endotoxin-induced uveitis by methylprednisolone acetate nanosuspension in rabbits

Adibkia, Khosro, Omidi, Yadollah, Siahi, Mohammad R, Javadzadeh, Ali R, Barzegar-Jalali, Mohammad, Barar, Jaleh, Maleki, Nasrin, Mohammadi, Ghobad and Nokhodchi, Ali (2007) Inhibition of endotoxin-induced uveitis by methylprednisolone acetate nanosuspension in rabbits. Journal of Ocular Pharmacology and Therapeutics, 23 (5). pp. 421-432. ISSN 1080-7683

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Abstract

In this study, nanoformulations of methylprednisolone acetate (MPA) were formulated by using a copolymer of poly(ethylacrylate, methyl-methacrylate and chlorotrimethyl-ammonioethyl methacrylate) to study their impacts on the inhibition of inflammatory symptoms in rabbits with endotoxin-induced uveitis (EIU).
A modified quasiemulsion solvent diffusion technique was used for the preparation of the nanoparticles. The drug-release profiles and physicochemical characteristics of the nanoformulations were studied by means of X-ray crystallography, differential scanning calorimetry, and Fourier transform infrared spectroscopy. Particle-size analysis yielded mean diameters of approximately 380, 460, and 580 (nm) for copolymer nanoparticles at the ratios of 1:2.5, 1:5, and 1:10, respectively. Major clinical symptoms of EIU (e.g., morphologic changes, leukocytes numbers, and protein levels within the aqueous humor) were examined.
Upon the physicochemical characterizations, no crystal changes or chemical interactions were observed for the copolymer nanoparticles. The 1:2.5 ratio of drug polymer resulted in the most controlled release of MPA. The in vivo examinations revealed that the endotoxin-induced inflammation can be inhibited by the copolymer nanosuspension more significantly than by the microsuspension of MPA itself in the rabbits with EIU.
Based on our findings, we suggest that the copolymer nanosuspension may favor the localized, controlled ocular delivery of MPA for the prevention of inflammatory symptoms in ocular diseases.

Item Type: Article
Schools and Departments: School of Life Sciences > Chemistry
Subjects: Q Science > QD Chemistry
Depositing User: Emma Ransley
Date Deposited: 06 Jan 2015 14:03
Last Modified: 06 Jan 2015 14:03
URI: http://sro.sussex.ac.uk/id/eprint/51722
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