Fast bi-exponential fluorescence lifetime imaging analysis methods

Li, David Day-Uei, Yu, Hongqi and Chen, Yu (2015) Fast bi-exponential fluorescence lifetime imaging analysis methods. Optics Letters, 40 (3). pp. 336-339. ISSN 0146-9592

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Abstract

A new hardware-friendly bi-exponential fluorescence lifetime imaging (FLIM) algorithm has been proposed. Compared to conventional FLIM software, the proposed algorithms are non-iterative offering direct calculation of lifetimes and therefore suitable for real-time applications. They can be applicable to single-channel or 2D multi-channel time-correlated single-photon counting (TCSPC) systems. The proposed methods have been tested on both synthesized and realistic FLIM data, and we have compared their performances with other recently proposed non-fitting bi-exponential techniques showing promising applications in future massive solid-state TCSPC imagers.

Item Type: Article
Keywords: Fluorescence lifetime imaging microscopy, photon counting, time-resolved fluorescence imaging
Schools and Departments: School of Engineering and Informatics > Engineering and Design
Subjects: Q Science > QA Mathematics > QA0273 Probabilities. Mathematical statistics
Q Science > QA Mathematics > QA0075 Electronic computers. Computer science
Q Science > QC Physics > QC0350 Optics. Light > QC0450 Spectroscopy
T Technology > TK Electrical engineering. Electronics Nuclear engineering > TK0452 Electric apparatus and materials. Electric circuits. Electric networks
Depositing User: David Day-Uei Li
Date Deposited: 03 Dec 2014 09:04
Last Modified: 08 Mar 2017 08:23
URI: http://sro.sussex.ac.uk/id/eprint/51569

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