A time-resolved multifocal multiphoton microscope for high speed FRET imaging in vivo

Poland, Simon P, Krstajić, Nikola, Coelho, Simao, Tyndall, David, Walker, Richard J, Devauges, Viviane, Morton, Penny E, Nicolas, Nicole S, Richardson, Justin, Li, David Day-Uei, Suhling, Klaus, Wells, Claire M, Parsons, Maddy, Henderson, Robert K and Ameer-Beg, Simon M (2014) A time-resolved multifocal multiphoton microscope for high speed FRET imaging in vivo. Optics Letters, 39 (20). pp. 6013-6016. ISSN 0146-9592

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Abstract

Imaging the spatio-temporal interaction of proteins in vivo is essential to understanding the complexities of biological systems. The highest accuracy monitoring of protein-protein interactions is achieved using FRET measured by fluorescence lifetime imaging, with measurements taking minutes to acquire a single frame, limiting their use in dynamic live cell systems. We present a diffraction limited, massively parallel, time-resolved multifocal multiphoton microscope capable of producing fluorescence lifetime images with 55 ps time-resolution, giving improvements in acquisition speed of a
factor of 64. We present demonstrations with FRET imaging in a model cell system and demonstrate in vivo FLIM using a GTPase biosensor in the zebrafish embryo.

Item Type: Article
Schools and Departments: School of Engineering and Informatics > Engineering and Design
Subjects: Q Science > QC Physics > QC0350 Optics. Light > QC0450 Spectroscopy
Depositing User: David Day-Uei Li
Date Deposited: 06 Oct 2014 09:50
Last Modified: 08 Mar 2017 08:30
URI: http://sro.sussex.ac.uk/id/eprint/50483

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