File(s) under permanent embargo
A time-resolved multifocal multiphoton microscope for high speed FRET imaging in vivo
journal contribution
posted on 2023-06-08, 18:36 authored by Simon P Poland, Nikola Krstajic, Simao Coelho, David Tyndall, Richard J Walker, Viviane Devauges, Penny E Morton, Nicole S Nicolas, Justin Richardson, David Day-Uei Li, Klaus Suhling, Claire M Wells, Maddy Parsons, Robert K Henderson, Simon M Ameer-BegImaging the spatio-temporal interaction of proteins in vivo is essential to understanding the complexities of biological systems. The highest accuracy monitoring of protein-protein interactions is achieved using FRET measured by fluorescence lifetime imaging, with measurements taking minutes to acquire a single frame, limiting their use in dynamic live cell systems. We present a diffraction limited, massively parallel, time-resolved multifocal multiphoton microscope capable of producing fluorescence lifetime images with 55 ps time-resolution, giving improvements in acquisition speed of a factor of 64. We present demonstrations with FRET imaging in a model cell system and demonstrate in vivo FLIM using a GTPase biosensor in the zebrafish embryo.
History
Publication status
- Published
File Version
- Published version
Journal
Optics LettersISSN
0146-9592Publisher
Optical Society of AmericaExternal DOI
Issue
20Volume
39Page range
6013-6016Department affiliated with
- Engineering and Design Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2014-10-06First Compliant Deposit (FCD) Date
2014-10-06Usage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC