Oleoyl-coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria

Turapov, Obolbek, Waddell, Simon J, Burke, Bernard, Glenn, Sarah, Sarybaeva, Asel A, Tudo, Griselda, Labesse, Gilles, Young, Danielle I, Young, Michael, Andrew, Peter W, Butcher, Philip D, Cohen-Gonsaud, Martin and Mukamolova, Galina V (2014) Oleoyl-coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria. Journal of biological chemistry, 289 (36). pp. 25241-25249. ISSN 1083-351X

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Abstract

Regulator of Antimicrobial-Assisted Survival), encoded by Rv1219c in Mycobacterium tuberculosis and bcg_1279c in Mycobacterium bovis BCG, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and to lesser extent palmitoyl-CoA) modulate RaaS binding to DNA and the expression of the genes downstream that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influenced RaaS-mediated mycobacterial improvement of survival and the expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used eliminate non-growing mycobacteria.

Item Type: Article
Schools and Departments: Brighton and Sussex Medical School > Global Health and Infection
Subjects: Q Science > QR Microbiology
Q Science > QR Microbiology > QR0075 Bacteria
Depositing User: Simon Waddell
Date Deposited: 05 Aug 2014 09:55
Last Modified: 15 Aug 2017 14:10
URI: http://sro.sussex.ac.uk/id/eprint/49466

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